INSTITUTE OF CYTOLOGY OF THE RUSSIAN ACADEMY OF SCIENCES

Group of Flow Cytometry and Sorting
    Head of the Group

    Valery V. ZENIN

    Ph.D.


Staff: two Ph.D. researchers


The Group was founded in 1989 within the Federal Scientific Technical Program "Human Genome" to solve problem of massive isolation of human chromosomes for the subsequent gene and DNA fragments mapping. At present the Group members are involved in works supported by grants of RFBR, Leading Scientific Schools' and Federal Programs. These works includes not only cell cycle analysis but also detection surface and intracellular antigens on different cell models (embryonic stem cells, stable cell lines, blood cells). The studies are carried out on flow cytofluorimeter EPIX XL (Backman Coulter). The group members undertakes efforts to develop protocols which allow widening the spectrum of research problems that can be solved by cytofluorimetric approach. For example, the device was adjusted for analysis of mitotic human chromosomes, for estimation of cells' growth characteristics.
Group members have an experience of work in forein labs. They also collaborate with other biological and medical institutions.


Main publications 2000-2010

Brichkina AI, Tararova ND, Aksenov ND, Pospelova TV. 2000. Function of an inhibitor of cyclin-dependent kinase p27/Kip in cells transformed by E1A + E1B19 kDa + E1A + cHa-Ras, differing in their ability to realize a G1-block during serum starvation. Tsitologiia. 42(12):1148-53.

Malashicheva AB, Kislyakova TV, Aksenov ND, Osipov KA, Pospelov VA. 2000. F9 embryonal carcinoma cells fail to stop at G1/S boundary of the cell cycle after gamma-irradiation due to p21WAF1/CIP1 degradation. Oncogene. 2000. 19(34):3858-65.

Brichkina AI, Tararova ND, Aksenov ND, Pospelov VA, Pospelova TV. 2001. Rat embryo fibroblasts transformed by complementation with oncogenes E1A+E1B-19 and E1A+cHa-ras differ in the ability to realize the G1/S block in serum free media. Tsitologiia. 43(11):1024-30.

Gamalei IA, Polozov IuS, Aksenov ND, Darieva ZA, Kirpichnikova KM, Pospelova TV. 2001. Cell cycle and formation of active form of oxygen in rodent fibroblasts. Tsitologiia. 43(6):602-12.

Gamalei IA, Polozov IuS, Kirpichnikova KM, Aksenov ND, Tararova ND, Pospelova TV. 2001. Distribution of rat embryonal fibroblasts through cell cycle phases in the presence of inhibitors of active oxygen species formation and N-acetylcysteine. Tsitologiia. 2001;43(7):633-8.

Kuznetsova AV, Kravatskii IuV, Aksenov AD, Shatrova AN, Zenin VV, Poletaev AI. 2001. A novel method of chromosome distribution analysis in saccharose density gradient. Biofizika. 46(4):619-24.

Smirnova IS, Yakovleva TK, Rosanov YM, Aksenov ND, Pospelova TV. 2001. Analysis of p53-dependent mechanisms in the maintenance of genetic stability in diploid tumourigenic line SK-UT-1B of human uterine leiomyosarcoma. Cell Biol Int. 25(11):1101-15.

Zenin VV, Klopov N.V., Drobchenko E.A., Aksenov N.D., Shatrova A.N., Poletaev A.I. 2001. Mitotic cell analysis by means of univariate flow cytometry. Biologicheskie membrany. 18(3): 207-215.

Kharchenko MV, Aksenov ND, Kornilova ES. 2002. Effect of a hypertonic sucrose solution and 5-(N,N-hexamethylene)-amiloride on receptor-mediated and liquid-phase endocytosis. Tsitologiia. 44(7):681-90.

Tararova ND, Bulavin DV, Neliudova AM, Aksenov ND, Pospelov VA, Pospelova TV. 2002. Anti-apoptotic and antiproliferative effect of bcl-2 gene transferred to E1A+cHa-ras-transformed cells. Tsitologiia. 44(5):441-9.

Shatrova AN, Aksenov ND, Zenin VV. 2002. Flow cytometric analysis of ICRF-193 influence on cell passage through mitosis. Tsitologiia. 44(11):1068-78.

Abramova MV, Svetlikova SB, Aksenov ND, Pospelova TV, Pospelov VA. 2003. Histone deacetylase inhibitor blocks proliferation of cells transformed with oncogenes E1A and cHa-ras Tsitologiia. 45(11):1100-8.

Brichkina AI, Aksenov ND, Pospelov VA, Pospelova TV. 2003. Analysis of transient G1/S arrest in E1A+E1B-19kDa transformed cells after ionizing radiation. Tsitologiia. 45(12):1203-10.

Gamalei IA, Aksenov ND, Efremova TN, Kirpichnikova KM. 2003. Effect of agents changing the intracellular level of reactive oxygen species on the cell cycle phase distribution in 3T3 and 3T3SV40 cell lines. Tsitologiia. 45(1):26-33.

Krylova TA, Zenin VV, Musorina NS, Baranov VS, Bichevaia NK, Korsak VS, Nikol'skii NN, Pinaev GP, Polianskaia GG. 2003. Isolation and characterisation of continuous human embryonic stem cell lines. Tsitologiia. 45(12):1172-8.

Marakhova II, Karitskaia II, Vinogradova TA, Aksenov ND, Moshkov AV, Khaidukova AL. 2003. Changes in sodium pump transport activity and Na+, K+-ATPase expression level following lymphocytes activation in humans. Tsitologiia. 45(11):1149-59.

Neliudova AM, Aksenov ND, Pospelova TV. 2003. Changes in the activity of cyclin-kinase complexes governing cell transition from G1 phase to DNA replication phase in E1A + c-Ha-ras transformants transfected with the bcl-2 gene. Tsitologiia. 2003;45(2):149-57.

Savel'eva IA, Bykova TV, Aksenov ND, Pospelov VA, Pospelova TV. 2003. MAP-kinase cascade analysis in transformed cell with different abilities to make G1/S block under serum starvation. Tsitologiia. 45(5):493-9.

Smirnova IS, Iakovleva TK, Aksenov ND. 2003. Delayed biological consequences induced by gamma-radiation in human tumorigenic diploid line SK-UT-1B. Tsitologiia. 45(4):422-7.

Shatrova AN, Aksenov ND, Poletaev AI, Zenin VV. 2003. Effect of etoposide and amsacrine on mitotic progression of GM-130 and Hep-2 cell lines. The flow cytometry assay. Tsitologiia. 45(1):59-68.

Goloudina AR, Aksenov ND, Pospelov VA. 2004. Degradation of Cdc25A phosphatase in HeLa cells under normal and stress conditions. Tsitologiia. 46(5):423-30.

Neliudova AM, Brichkina AI, Rukhlova MP, Aksenov ND, Pospelova TV. 2004 Antiproliferative effect of bcl-2 gene does not concern the control of mitotic events. Tsitologiia.46(3):257-67.

Nelyudova AM, Tararova ND, Aksenov ND, Pospelov VA, Pospelova TV. 2004. Restoration of G1/S arrest in E1A+c-Ha-ras-transformed cells by Bcl-2 over expression. Cell Cycle. 3(11):1427-32.

Nikitina TV, Nazarova NY, Aksenov ND, Tishchenko LI, Tuohimaa P, Sedova VM. 2004. Small stable RNA level depends on the physiological state of the cell. Tsitologiia. 46(5):437-41.

Karitskaia IA, Aksenov ND, Vinogradova TA, Marakhova II. 2005. Il-2-regulated expression of Na+,K(+)-ATPase in activated human lymphocytes. Tsitologiia. 47(1):28-37.

Krylova TA, Zenin VV, Mikhailova NA, Pinaev GP, Nikol'skii NN, Polianskaia GG. 2005. Continuous human embryonic stem cell lines. Tsitologiia. 47(2):121-9.

Marakhova I, Karitskaya I, Aksenov N, Zenin V, Vinogradova T. 2005. Interleukin-2-dependent regulation of Na/K pump in human lymphocytes. FEBS Lett. 579(13):2773-80

Neliudova AM, Zubova SG, Aksenov ND, Pospelov VA, Pospelova TV. 2005. Antiapoptotic oncogene bcl-2 induces a program of senescence in E1A + c-Ha-ras-transformants treated with adriamycin. Tsitologiia. 47(10):907-16.

Zubova IuG, Bykova TV, Zubova SG, Abramova MV, Aksenov ND, Pospelov VA, Pospelova TV. 2005. Induction of premature senescence program by an inhibitor of histone deacetylase sodium butyrate in normal and transformed rat fibroblasts. Tsitologiia. 47(12):1055-62.

Karitskaia IA, Aksenov ND, Zenin VV, Khaidukova AL, Marakhova II. 2006. Activation of transcriptional factors stat5 and stat3 during human T-lymphocyte proliferation induced by different mitogenic stimuli. Tsitologiia. 48(1):42-9.

Mikhailov VM, Evtifeeva EV, Serikov VB, Perverzev AE, Karmanova AV, Zenin VV. 2006. Participation of bone-marrow stem cells in the differentiation of mdx mice striated muscle. Tsitologiia. 48(5):410-7.

Smirnova IS, Aksenov ND, Kashuba EV, Payakurel P, Grabovetsky VV, Zaberezhny AD, Vonsky MS, Buchinska L, Biberfeld P, Hinkula J, Isaguliants MG. 2006. Hepatitis C virus core protein transforms murine fibroblasts by promoting genomic instability. Cell Oncol. 28(4):177-90.

Smirnova IS, Aksenov ND, Vonsky MS, Isaguliants MG. 2006. Different transformation pathways of murine fibroblast NIH 3T3 cells by hepatitis C virus core and NS3 proteins. Cell Biol. 30(11):915-9.

Grudinkin PS, Zenin VV, Kropotov AV, Dorosh VN, Nikolsky NN. 2007. EGF-induced apoptosis in A431 cells is dependent on STAT1, but not on STAT3. Eur. J. Cell Biol. 2007. 86(10):591-603.

Kuznetsova IS, Enukashvily NI, Noniashvili EM, Shatrova AN, Aksenov ND, Zenin VV, Dyban AP, Podgornaya OI. 2007. Evidence for the existence of satellite DNA-containing connection between metaphase chromosomes. J Cell Biochem. 101(4):1046-61.

Nelyudova A, Aksenov N, Pospelov V, Pospelova T. 2007. By blocking apoptosis, Bcl-2 in p38-dependent manner promotes cell cycle arrest and accelerated senescence after DNA damage and serum withdrawal. Cell Cycle. 6(17):2171-7.

Nevzgliadova OV, Artemov AV, Zenin VV, Verkhusha VV, Shavlovskii MM, Povarova OI, Stepanenko OV, Kuznetsova IM, Turoverov KK. 2007. Expression of recombinant actin 5C from Drosophila in the methylotrophic yeast Pichia pastoris. Tsitologiia. 49(4):300-10.

Rider L, Shatrova A, Feener EP, Webb L, Diakonova M. 2007. JAK2 tyrosine kinase phosphorylates PAK1 and regulates PAK1 activity and functions. J Biol Chem. 282(42):30985-96

Shamova OV, Sakuta GA, Orlov DS, Zenin VV, Shtein GI, Kolodkin NI, Afonina IV, Kokriakov VN. 2007. Effects of antimicrobial peptides of neutrophils on tumor and normal cells in culture. Tsitologiia. 49(12):1000-10.

Zubova SG, Bykova TV, Zubova IuG, Romanov VS, Aksenov ND, Pospelov VA, Pospelova TV. 2007. Role of p38alpha kinase in activation of premature senescence program in transformed mouse fibroblasts. Tsitologiia. 49(2):115-24.

Karitskaia IA, Aksenov ND, Vasil'eva IO, Strelkova EG, Marakhova II. 2008. [Long-term regulation of Na+, K+-ATPase pump in human lymphocytes: the role of JAK/STAT- and MAPK-signaling pathways. Tsitologiia. 50(4):329-37.

Zubova SG, Bykova TV, Zubova IuG, Romanov VS, Aksenov ND, Pospelov VA, Pospelova TV. 2008. Sodium butyrate do not induce the program of premature senescence in transformants with JNK1,2 knockout. Tsitologiia. 50(11):964-71.

Bukreeva EI, Aksenov ND, Bardin AA, Pospelov VA, Pospelova TV. 2009. Effect of histone deacetylases inhibitor sodium butyrate (NaB) on transformants E1A + cHa-Ras expressing wild type p53 with suppressed transactivation function. Tsitologiia. 51(8):697-705.

Kozhukharova IV, Fridlianskaia II, Kovaleva ZV, Pugovkina NA, Alekseenko LL, Zenin VV, Ivantsov KM, Leont'eva OK, Grinchuk TM, Nikol'skii NN. 2009. Novel human embryonic stem cell lines C612 and C910. Tsitologiia. 51(7):551-8.

Krylova TA, Kol'tsova AM, Zenin VV, Gordeeva OF, Musorina AS, Goriachaia TS, Shlykova SA, Kamenetskaia IuK, Pinaev GP, Polianskaia GG. 2009. The characters and specific features of new human embryonic stem cells lines. Tsitologiia. 51(7):565-76.

Polubotko EA, Shatrova AN, Pleskach NM, Mikhel'son VM, Spivak IM. 2009. Cellular repair potential in families of ataxia-telangiectasia patients. Tsitologiia. 51(12):978-85.

Vakhromova EA, Polozov IuS, Kirpichnikova KM, Aksenov ND, Gamalei IA. 2009. Effect of alpha-lipoic acid on fibroblasts 3T3 and 3T3-SV40. Comparison with N-acetylcysteine action. Tsitologiia. 51(12):971-7.

Zenin VV, Aksenov ND, Shatrova AN, Marakhova II. 2009. The expression of CD25 in phytohemagglutinin- or interleukin-2-stimulated human peripheral blood lymphocytes. Tsitologiia. 51(6):506-10.

Butylin P.A., Zenin V.V., Sidorkevich S.V. 2010. Human platelet-monocyte in vitro interaction test using flow cytometry. In: "Translacionnaya medicina". Ad. Prof. E.B. Shlyakhto. St.Petersburg. Russia. p. 376-85.

Karitskaya I, Aksenov N, Vassilieva I, Zenin V, Marakhova I. 2010. Long-term regulation of Na,K-ATPase pump during T-cell proliferation. Pflugers Arch. 460(4):777-89.

Kozhucharova I. V., Fridlyanskaya I. I., Zemelko V. I., Kovaleva Z. V., Pugovkina N. A., Aleksecnko L. L., Charchenko M. V., Shatrova A. N., Akscnov N. D., Grinchuk . ., Anisimov S. V., Nikolsky N. N. 2010. Generation of dopamine neurons from human embryonic stem cells in vitro. Tsitologiya. 52 (10):875-882.

Mitiushova EV, Aksenov ND, Marakhova II. 2010. Specific function of STAT5 in regulation of proliferation of chronic leukemia K562 cells: inhibitory effect of WHI-P131. Tsitologiia. 52(2):184-90.

Romanov VS, Abramova MV, Svetlikova SB, Bykova TV, Zubova SG, Aksenov ND, Fornace AJ Jr, Pospelova TV, Pospelov VA. 2010. p21(Waf1) is required for cellular senescence but not for cell cycle arrest induced by the HDAC inhibitor sodium butyrate. Cell Cycle. 9(19): 3945-55.

Smirnova IS, Gonchar IV, Shatrova AN, Nikol'skii NN, Burova EB. 2010. Metalloprotease-mediated HB-EGF release regulates EGF receptor transactivation in A431 cells under oxidative stress. Tsitologiia. 52(5):357-63.

Sokolova AV, Zenin VV, Mikhailov VM. 2010. Structure of neuromuscular junctions and differentiation of striated muscle fibers of mdx mice after bone marrow stem cells therapy. Tsitologiia. 52(5):399-406.

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