INSTITUTE OF CYTOLOGY OF THE RUSSIAN ACADEMY OF SCIENCES

Confocal Microscopy and Image Analysis Group
Head of the Group

Grigory I. STEIN

Leading Scientist, Ph.D.

phone: (812) 297-13-13
e-mail: spbgistn@mail.ru

The Group was organized in 2002 to investigate scientific and methodological problems of confocal microscopy and image analysis, also to help researchers of Institute in their microscopic studies. Now the Group has laser scanning confocal microscopes OlLYMPUS FV3000, LEICA TCS SP5, fluorescent microscopes AXIOVERT 200M, AXIOPHOT and AXIOSKOP with digital videosystems.


M.L. Vorobjev

Scientific Staff:

S.V. Zherebtsov, Junior Research Scientist, Ph.D.

M.L. Vorobjev, Leading Engineer





Comparative features of laser scanning confocal microscopes

Features


   Microscope
   Objectives
   
   Temperature control
   Laser lines (nm)
   Main beamsplitter
   Spectral confocal channels
   Max frame resolution (pixel)
   Scan zoom
   Max line frequency (lin/s)
   Resonant scanner (lin/s)
   3D visualization
   Spectral scanning
   Colocalization
   FRAP
   FRET
   Manufacturing

Leica TCS SP5


   inverted
   10×/0.4, 20×/0.7; 40×/1.25,
   100×/1.4, 40×/0.55 (LD)
   ¬ox
   405, 458, 488, 514, 543, 633
   AOBS
   3 PMT (+BF/DIC)
   8192×8192
   1−64
   2800
   16 000
   +
   +
   +
   +
   AB
   2008

Olympus FV3000


   inverted
   10×/0.3, 20×/0.5, 20×/0.45(LD),
   40×/1.3, 40×/0.6 (LD), 60×/1.42
   Box/stage
   405, 488, 561, 640
   dichroic mirrors
   2 PMT+2 GaAsP (+BF/DIC)
   4096x4096
   1−50
   2000
   16 000
   +
   +
   +
   +
   SE
   2016

Selected Publications:

Bogolyubova I., Stein G., Bogolyubov D. FRET analysis of interactions between actin and exon-exon junction complex proteins in early mouse embryos. Cell Tissue Res. 2013. 352: 277-285.

Agafonova N. A., Sakuta G.A., Rozanov Yu. M., Shtein G.I., Kudruavtsev B.N. DNA image-fluorimetry of individual human chromosomes. Cell and Tissue Biology. 2013. 7(4): 352-361.

Myasnikova E., Surkova S., Stein G., Pisarev A., Samsonova M. Regression system for estimation of errors introduced by confocal imaging into gene expression data in situ. BMC Bioinformatics. 2011. 12: 20-331.

Bezborodkina N.N., Shtein G.I., Sivova E.V., Chestnova A.Yu., Kudryavtsev B.N. Analysis of structure of glycogen in rat hepatocytes using cytochemical and FRET methods. Cell and Tissue Biology. 2011. 5(5): 417-427.

Egorova O.V., Shtein G.I. A comparision of fluorescence-microscope illuminator systems based on LEDs and HBO mercury lamps. J.Opt.Technol. 2011. 78(1): 81-83.

Stein G.I. Confocal microscopy and its application for analysis of cell cultures. In: Methods of cell cultivation (Eds. G. Pinaev, M. Bogdanova). Publ. SPbSPU, 2008. p.261-274.

Stein G.I. Manual of confocal microscopy. Saint-Petersburg, Publ. SPbSPU, 2007, 77p.

Stein G.I. Resolution of confocal microscope: evolution of ideas. 2006.

Stein G.I. Confocal microscopy: myths and reality. 2005. 

Stein G.I. Laser scanning confocal microscopy. Manual. Publ. ICRAS. 2004. 32p.

Stein G.I. The influence of image analyser noises on the image quality in studies of cell texture features. Tsitologiya. 2002. 44 (1) : 102-106.

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