The Group of Genome Microevolution and Cytoecology
    Head of the Group

    Yu. M. ROZANOV

    PhD, principal research associate

    Tel.: (812) 297-53-10

    The group was established in 2005 by joining of Group of evolutionary cytoecology and biomonitoring (Laboratory of cytology of protozoan organisms) with Group of fluorescent microscopy and cytometry (Laboratory of biochemical cytology and cytochemistry).

    The scientific aims of the group are peculiarities of genome size variation, mechanisms of adaptive genome size variability, "C-value enigma", significance of noncoding DNA in evolution. The group studies various groups of vertebrates: amphibians, reptiles and mammals.

Group Membership:

S.N.Litvinchuk, PhD, senior research associate
D.V.Skorinov, PhD, research associate
R.A.Pasynkova, research associate

Areas of Investigations

The analysis of intraspecific variability of the nuclear DNA content. The study of relationships between genome size differences and levels of divergence between taxa.

The study of correlation of the nuclear DNA content and various geno- and phenotypic characters, such as allozyme and microsatellite frequencies, levels of genome heterochromatization, morphological (metric) peculiarities, termoresistance of various tissues, and time of embryonic development (on the example of amphibians).

The analysis of variation of the nuclear DNA content and peculiarities of inheritance in hybridogenic complexes in amphibians.

Development and upgrading of methods and equipment for precise DNA cytometry using for determination of genome size in somatic and sexual cells.

We studied the nuclear DNA content in various groups of vertebrates. In total we studied more than 110 species of amphibians and reptiles of Russia (European, Siberian, and Far Eastern parts), Kazakhstan, Middle Asia, the Caucasus, Ukraine, Western Europe, Mediterranean countries (Turkey, Tunisia, Cyprus, Greece) and Japan. We received unique data about intraspecific variation of genome size in Bufonidae, Ranidae, Hylidae, Salamandridae, Hynobiidae, Bombinatoridae, Pelobatidae, etc. Huge number of studied samples allowed us suggests that in some groups (for example in amphibians) the nuclear DNA amount is most universal and reliable character for detection of events of early divergence in comparison with such genotypic and phenotypic features as allozyme and microsatellite frequencies, levels of genome heterochromatization, and morphometric characters.

Main Publications (1986-2007):

Borkin L.J., Caune I.A., Pisanetz E.M., Rozanov Y.M. 1986. Karyotype and genome size in the Bufo viridis group. In: Studies in Herpetology, Rocek Z.(ed), Prague, pp. 137-142. Darevsky I.S., Danielyan F.D., Sokolova T.M., Rozanov Y.M. 1989. Intraclonal mating in the parthenogenetic lizard species Laсerta unisexualis Darevsky. In: Evolution and Ecology of Unisexual Vertebrates. R. W. Dawley and J. P. Bogart (Eds.), New York State Museum, Albany, New York, USA, pp. 228-235. Vinogradov A.E., Borkin L.J., Gunther R., Rosanov J.M. 1990. Genome elimination in diploid and triploid Rana esculenta males : cytological evidence from DNA flow cytometry. Genome. 33 : 619-627. Vinogradov A.E., Borkin L.J., Gunther R., Rosanov J.M. 1991. Two germ cell lineages with genomes of different species in one and the same animal. Hereditas. 114 : 245-251. Litvinchuk S.N., Rosanov J.M., Borkin L.J. 1997. A contact zone between the newts Triturus cristatus and Triturus dobrogicus in the Ukrainian Transcarpathians: distribution and genomt size variation. In: Herpetologia bonnensis, published by Societas Europian Herpetologica (SEN), W.Bohme, W. Bischoff, T. Ziegler (eds), Bonn, pp. 229-235. Rozanov Yu.M , Vinogradov A.E. 1998. The precise DNA-cytometry : investigation of the individual variability of the animal genome size. Tsitologiya. 40 (8/9) : 792-800 (In Russian). Borkin L.J., Rosanov J.M., Litvinchuk S.N. 2000. Nuclear DNA content in some green toads (Bufo viridis complex) of Turkey and Iran. Russian J. Herpetology. 7 (3) : 171-180. Borkin L. J., Litvinchuk S. N., Milto K. D., Rosanov J. M., Khalturin M. D. 2001. Cryptic speciation in Pelobates fuscus (Anura, Pelobatidae): cytometrical and biochemical evidences. Doklady Biological Sciences, 376: 86-88. Borkin L.J., Litvinchuk S.N., Milto K.D., Rosanov J.M. 2001. Criptic speciation in Pelobates fuscus: evidence from DNA flow cytometry. Amphibia-Reptilia, 22 : 419-430. Litvinchuk S.N., Borkin L.J., Rosanov J.M. 2003. On distribution of and hybridization between the newts Triturus vulgaris and T. montandoni in western Ukraine. Alytes, Paris. 20 : 161-168. Khalturin M. D., Litvinchuk S. N., Borkin L. J., Rosanov J. M., Milto K. D. 2003. Genetic variation in two cryptic forms, with different genome size, of the common spadefoot toad, Pelobates fuscus (Pelobatidae, Anura, Amphibia). Tsitologia, 45 (3): 308-323 (in Russian). Litvinchuk S.N., Borkin L.J., Rosanov J.M. 2004. Intraspecific and interspecific genome size variation in hynobiid salamanders of Russia and Kazakhstan: determination by flow cytometry. Asiatic Herpetol. Res., Berkeley, 10 : 282-294. Borkin L. J., Litvinchuk S. N., Rosanov J. M., Skorinov D. V. 2004. On cryptic species (from the example of amphibians). Zoologichesky Zhurnal, 83 (8): 936-960 (in Russian; translated in English as Entomological Review, 84 (Suppl. 1): S75-S98). Litvinchuk S.N., Zuiderwijk A., Borkin L.J., Rosanov J.M. 2005. Taxonomic status of Triturus vittatus (Amphibia: Salamandridae) in western Turkey: trunk vertebrae count, genome size and allozyme data. Amphibia-Reptilia, Leiden. 26 : 305-323. Bogdanov A.S., Rozanov Yu.M. 2005. Variability in size of the nuclear genome in pygmy wood mouse Sylvaemus uralensis (Rodentia, Muridae). Russian J. Genetics. 41 (10) : 1123-1129 (in Russian). Weisrock D.W., PapenfussT.J., Macey J.R., Litvinchuk S.N., Polymeni R., Ugurtas I.U., Zhao E., Jowkar H., Larson A. 2006. A molecular assessment of phylogenetic relationships and lineage accumulation rates within in the family Salamandridae (Amphibia, Caudata). Molecular Phylogenetics and Evolution, 41: 368 - 383. Litvinchuk S.N., Pashkova I.M., Rosanov J.M., Borkin L.J. 2007. Heat resistance of the muscle in West-Palearctic green frogs of Rana esculenta complex. Biology Bulletin, 1: 61-66. Litvinchuk S.N., Rosanov J.M., Usmanova N.M., Borkin L.J., Mazanaeva L.F., Kazakov V.I. 2007. Variability of microsatellites BM224 and Bcal7 in populations of green toads (Bufo viridis complex) differing by nuclear DNA content and ploidy. Cell and Tissue Biology, 1 (1): 65-79. Litvinchuk S. N., Rosanov J. M., Borkin L. J. 2007. Correlations of geographic distribution and temperature of embryonic development with the nuclear DNA content in the Salamandridae (Urodela, Amphibia). Genome, 50: 333-342.

Structure    Home