INSTITUTE OF CYTOLOGY OF THE RUSSIAN ACADEMY OF SCIENCES

Group of ˝ells biotechnology

    Group Leader

    Dr. Miralda I. BLINOVA, Ph.D.

    Phone: +7(812) 297-39-83
    Fax: +7 (812) 297-03-41
    e-mail: mira.blinova@mail.ru


Main Staff of the Group

Dr. Natalia Yudintceva, Ph.D.
yudintceva@mail.ru

Dr. Yulia Nashchekina, Ph.D.
ulychka@mail.ru

Dr. Svetlana Alexandrova, Ph.D.
alekssvet2205@gmail.com

Dr. Diana Darvish, Ph.D.
darvishdi@mail.ru


Research Assistant

Olga Alexandrova
elga.aleks@gmail.com

Pavel Nikonov
pashka2316@mail.ru

Konstantin Kolobov
kostyakolobok@rambler.ru


Students

Yulia Khorolskaja
juliya_khorolskaya@mail.ru

Pavel Kopelev
paxa94@bk.ru

Elizaveta Kasjanova
k_elisa@bk.ru

Alena Kashirova
k-alyona94@ya.ru

Victoria Karpovich
kviccy@gmail.com

Ekaterina Chizhikova
17.chizh@gmail.com


Research Interests

The leading trend of the group is the creating of scientific bases of cellular biotechnology and the development of new high-tech biomedical technologies of regenerative medicine. The technology of using of the cultivated cells of human skin (keratinocytes and fibroblasts) for restore of the different etiology wounds was developed.

A number of cell products were created:

  • Dermal equivalent is based on the collagen Type I (collagen gel with the fibroblasts);
  • Dermal equivalent is based on the human plasma fibrin (fibrin gel with the fibroblasts);
  • Multilayer sheet of the keratinocytes;
  • Multilayer skin equivalent (the first layer is the collagen gel containing dermal fibroblasts and second layer is the sheet of the keratinocytes)

The permission of the Ministry of Health of the Russian Federation for serial production and clinical application of the dermal equivalent is based on the collagen Type I and the multilayered sheet of the keratinocytes have been received.

The cell products have been tested on clinical practice for the healing wounds of different etiology: burns, venous and diabetic ulcers, fistula (Crohn's disease, gunshot wounds), bedsores etc. They were applied into the leading clinics of the Saint-Petersburg: Saint-Petersburg I.I. Gzhanelidze Research Institute of Emergency Medicine, S.M. Kirov Army Medical Academy, Saint-Petersburg Hospital for Veterans of War, Saint-Petersburg Hospital ╣9 and other hospitals.
Dermal fibroblasts were cultured on the porous polytetrafluoroethylene membrane with a positive result have been used in ophthalmology for scleroplasty (Saint-Petersburg Hospital ╣2).

Nowadays the experimental possibilities of using the bone marrow stromal cells cultured on the biodegradable 3D scaffolds to restore the damage of the bladder and the application of the limbal cells to restore the cornea are investigated.
The one of the direction of the investigations is the research of the inducted differentiation of the bone marrow stromal cells into osteogenic and chondrogenic directions in order to use them for regeneration of bone and cartilage tissues.
Another direction of our works is the isolation from different tissue the extracellular proteins such as collagen of various types, laminin, fibronectin and other once for research and clinical applications.


Methods
Traditional methods of cell biology including isolation of cells from different tissues of human and animals, cultivation of normal, immortalized and cancer cells into the 2D and 3D conditions, analysis structure and properties of 3D scaffolds, confocal immunoflurescence.

Financial support
    Current financial support
  • Russian Science Foundation grant 14-50-00068
  • Russian Foundation for Basic Research grant 15-29-04852
    Past financial support
  • Russian Foundation for Basic Research grant 13-04-12027 ofi_m (2013-2015)

Scientific collaborations
  • Saint-Petersburg I.I. Gzhanelidze Research Institute of Emergency;
  • Medicine, S.M. Kirov Army Medical Academy;
  • First I.P.Pavlov State Medical University of St.Petersburg;
  • Federal Almazov North-West Medical Research Center;
  • St.Petersburg Scientific-Research Institute of Phthisiopulmonology, Russian Ministry of Health Care;
  • The Federal State Budgetary Institute "The Nikiforov Russian Center of Emergency and Radiation Medicine". The Ministry of Russian Federation for Civil Defense, Emergencies and Elimination of Consequences of Natural Disasters;
  • Institute of Marcomolecular Compounds RAS;
  • Sharon, MA, USA and Tufts University School of Medicine, Boston, MA, USA

Main publications (2007-2016)
  1. Pitkin M., Raykhtsaum G., Pilling J., Galibin O.V., Protasov M.V., Chihovskaya J.V., Belyaeva I.G., Blinova M.I., Yudintseva N.M., Potokin I.L., Pinaev G.P., Moxson V., Duz V. 2007. Porous composite prosthetic pylon for integration with skin and bone. Journal of Rehabilitation Research and Development, V. 44 (5), p.723-38.
  2. Shved Yu.A., Kukhareva L.B., Zorin I.M., Bilibin A.Yu., Blinova M.I., Pinaev G.P. 2007. Interaction of cultured skin cells with the polylactide matrix coved with different collagen structural isoforms. Cell and Tissue Biology. V. 1(1), p.89-95.
  3. Protasov M.V., Smagina L.V., Yudintseva N.M., Galibin O.V., Pinaev G.P., Voronkina I.V. 2009. Possibility of predicting rat wound epithelization by changes in matrix metalloproteinases activities in wound exudate. Cell and Tissue Biology. V. 3 (3), p.249-253.
  4. Aksakal B., Tsobkallo E.S., Darvish D. 2009. Mechanical properties of Bombix mori silk yarns studied with tensile testing method. Applied Polymer Science, V. 113, p.2514 2523.
  5. Turoverova L.V., Khotin M.G., Yudintseva N.M., Magnusson K.-E., Blinova M.I., Pinaev G.P., Tentler D.G. 2009. Analysis of extracellular matrix proteins produced by cultured cells. Tsitologiya. V. 51 (8), p.691-696.
  6. Gorshkov A.N., Blinova ╠.I., Pinaev G.P. 2009. Ultrastructure of coelomic epithelium and coelomocytes of intact and wounded starfish asterias rubens. Tsitologiya. V. 51 (8): p.650-661.
  7. Yudintseva N., Pleskach N., Smagina L., Blinova M., Samusenko, I., Pinaev G. 2010. Reconstruction of the connective tissue as a result of transplantation of fibrins dermal equivalent to the wounds of experimental animals. Tsitologiya. V. 52 (9), p.724-728.
  8. Yudintseva, N.M., Pleskach, N.M., Smagina, L.V., Blinova, M.I., Samusenko, I.A., Pinaev, G.P. 2010. Reconstruction of Connective Tissue from Fibrin-Based Dermal Equivalent Transplanted to Animals with Experimental Wounds. Cell and Tissue Biology. V.4 (5), p.476-480.
  9. Tsobkallo E.S., Aksakal B., Darvish D.M. 2010. Recovery processes in stretched wool fibres, Journal of Macromolecular Science, Part B Physics. V. 49 (3), p.495 505.
  10. The cultivation of cells on the porous titanium implants with the different structure Blinova M.I., Yudintzeva N.M., Nikolaenko N.S., Potokin I.L., Raykhtsaum G., Pitkin M., Pinaev G.P. 2010. V.52(10): p.835-843.
  11. Goryukhina O.A., Martyushin S.V., Blinova M.I., Poljanskaya G.G., Cherepanova O.A., Pinaev G.P. 2010. Cultivation of cells on a surface covered by microspheres with coupled histones. 2010. V.52 (1), p.12-23.
  12. Raydan M., Shubin N.A., Blinova M.I., Prokhorov G.G., Pinaev G.P. 2011. The effect of low temperatures on the viability of human epidermal keratinocytes found at different stages of differentiation. Tsitologiya. V. 53 (1), p.22-30.
  13. Raydan M., Shubin N.A., Nikolaenko N.S., Blinova M.I., Prokhorov G.G., Pinaev G.P. 2011. Stability of bone marrow stromal cells to low temperatures according to their degree of differentiation. Tsitologiya. V. 53 (3), p.221-226.
  14. Aksenova V.Yu., Kholin M.G., Twoverova L.V., Yudintseva N.M., Magnusson K.-E., Pinaev G.P., Tentler D.G. 2012. Novel splicing isoform of actin-binding protein alpha-actinin 4 in epidermoid carcinoma cells A431. Tsitologiya. V. 54 (1), p.25-32.
  15. Katherina Tsobkallo, Baki Aksakal, Diana Darvish. 2012. Analysis of the contribution of the microfibrils and matrix to the deformation processes in wool fibers, Applied Polymer Science. V.125 (S2), p. E168-E179.
  16. Yudintseva N.M., Nikolaenko N.S., Voronkina I.V., Smagina L.V., Pinaev G.P. 2013. Migration rate of rabbit bone-marrow stromal cells and rabbit dermal fibroblasts in different gels and activity of their MMPS. Cell and Tissue Biology. V. 7 (5), p.426-432.
  17. Alexandrova S.A., Pinaev G.P. 2014. Actin cytoskeleton reorganization in bone marrow multipotent mesenchymal stromal cells at the initial step of transendothelial migration. Biophysics. V. 59 (5), p.741-745.
  18. Yu. A. Nashchekina, P. O. Nikonov, V. M. Mikhailov, G. P. Pinaev. 2014. Distribution of Bone-Marrow Stromal Cells in a 3D Scaffold Depending on the Seeding Method and the Scaffold Inside a Surface Modification. Cell and Tissue Biology. V. 8 (4), p.313-320.
  19. Nashchekina Yu.A.,Zorin I.M., Fetin P.A., Skachilova S.Yu., Bilibin A.Yu. 2014. Composite film coatings based on poly (D,L-lactide) and acexamic acid. Russian Journal of Applied Chemistry. V.87 (8), p.1146-1150.
  20. Shevtsov M.A., Galibin O.V., Yudintceva N.M., Blinova M.I., Pinaev G.P., Ivanova A.A., Savchenko O.N., Suslov D.N., Potokin I.L., Pitkin E., Raykhtsaum G., Pitkin M.R. 2014. Two-stage implantation of the skin- and bone-integrated pylon seeded with autologous fibroblasts induced into osteoblast differentiation for direct skeletal attachment of limb prostheses. J Biomed Mater Res Part A: 102A, p.3033-3048.
  21. Shevtsov M, Yudintceva N, Blinova M, Pinaev G, Galibin O, Potokin I, Popat I, Pitkin M. 2015. Application of the skin and bone integrated pylon with titanium oxide nanotubes and seeded with dermal fibroblasts. Prosthetics and Orthotics International. V.39 (6), p.477-486.
  22. Bildyug N.B., Voronkina I.V., Smagina L.V.,Yudintseva N.M., Pinaev G.P. 2015. Matrix metalloproteinases in primary culture of cardiomyocytes. Biochemistry. V.80 (10), p.1318-1326.
  23. Yudintceva N.M, Nazhchekina Y.A, Blinova M.I, Orlova N.V, Muraviov A.N, Vinogradova T.I, Sheykhov M.G, Shapkova E.Y, Emeljannikov D.V, Yablonskii P.K, Samusenko I.A, Mikhrina A.L, Pakhomov A.V, Shevtsov M.A. 2016.Experimental bladder regeneration using poly-L-lactide/silk fibroin (PL-SF) scaffold seeded with nanoparticle labelled allogenic bone marrow stromal cells. International Journal of Nanomedicine. V.11, p.4521-4533.
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