INSTITUTE OF CYTOLOGY OF THE RUSSIAN ACADEMY OF SCIENCES

Group of Cell Population Genetics
    Head of the Group

    Viacheslav M. MIKHAILOV

    Professor, PhD, DSc

    Member of the International Society of Differentiation, St. Paul, Minnesota, USA

    Phone: (812) 2971846
    e-mail: vmikhailov@mail.cytspb.rssi.ru




Members:

Anastasija Sokolova
Elena Kaminskaja
Antonina Mironova


Interlaboratory Group is the scientific follower of the Laboratory "Cell Population Genetics", founded and headed by Professor Y. B. Vakhtin in 1978-2006. The contribution of Prof. Y. B. Vakhtin and his assistants to the development of somatic cells genetics in Russia can not be overestimated. His books "Genetics of Somatic Cells" (1974) and "Genetic Theory of Cell Populations" (1980) laid the foundation of genetics of somatic cells and epigenetic heredity in Russia. The development of somatic cells genetics was used as the basis for creation of clonal-selectional theory of oncogenesis exposed comprehensively in his monograph "Clonal Selection Theory of Neoplasm" (1987). Prof. Vachtin was an editor of his students books, I. N. Shvemberger (1976) and of A. E. Pereverzev (1986) devoted to the biology of cancer and stem cells. The creation of transplantable strains of rat rhabdomyosarcomas RA-23 with extremely high metastasizing property is one of practical achievements of Y. B. Vakhtin. At 1995 RA-23 cells was patented as "The strain of tumor rhabdomyosarcoma" and has been used as a model for studying the processes of organotypic metastasizing and screening of antitumor drugs.

At present Group's work is focused on molecular and genetic research of the main events in the cell population life cycles, functioning of stem cells and survival of terminally differentiated cells. Program of investigations includes the studies of heterogeneity of bone marrow stem cell populations and their use for cell therapy. There was shown that local or general stem cell therapy of chimeric mdx mice repairs the structure and function of nerve-muscle junctions. Reparation of NMJs structure is accompanied by recovery of membrane resting potential more expressed in the end-plate region. The next direction is the epigenetic and genetic mechanisms of malignant cell differentiation. And the last one is the study of individual level of tolerance and their variability of different kinds of cell populations after stress action of ecological factors. To-day the Group's efforts are concentrated upon participation of DNA reparation in the survival of tissue cells first of all of cardiomyocytes. Objects are skeletal muscle fibers and cardiomyocytes of mutant mdx mice, human and animal decidual cells, lung, kidney and nerve cells. The establishment of age-related sensitivity of chondrocytes of bone growth zones DNA synthesis to the action of impulse magnet field and ion cyclotron resonance is another Group's achievement. Result have become a theoretical ground for the magnetic fields applications in clinical practice for noninvasive treatment of idiopathic scoliosis and other children's bone disorders in collaboration with hospital "Ogonyok's Recovery Center of Child's Orthopaedics and Traumatology". (St. Petersburg). Russian Federation patent 2275943 "The way of treatment of idiopathic scoliosis" was obtained by Prof V. M. Mikhailov and co-workers in 2006. In 2009 for this work Prof V. M. Mikhailov and colleagues have been took by Kosygin's award of Russian Association of Commodity Producers for innovation of noninvasive methods in cue of bone diseases in pediatrics. Prof. V. M. Mikhailov is member of Interional Society of Differentiation (St. Paul, Minnesota, USA).


Main Publications:

  1. Vakhtin Y.B. 1974. Genetics of Somatic Cells. Leningrad, Nauka, 258 pp.
  2. Shvemberger I.N. 1976. Cancer and Cell Differentiation. Leningrad, Nauka, 159 pp.
  3. Vakhtin Y.B. 1980. Genetic Theory of Cell Populations. Leningrad, Nauka, 168 pp.
  4. Pereverzev A. E. 1986. Haematopoietic colony-forming cells and physical stress factors. Leningrad, Nauka, 172 pp.
  5. Vakhtin Y.B., et al. 1987. Clonal-selectional concept of tumor growth. Kiev, Naukova Dumka, 216 pp.
  6. Kaminskaya E.V. et al. 1990. Instability of karyotype in offspring of truly malignant cells of rat rhabdomyosarcoma RA-2. Dokl. AN USSR. 310 (1): 207-210.
  7. Baranov A., Glazkov P., Mikhailov V. et al., 1999. Local and distant transfection of mdx mice fibers with dystrophin and LacZ genes delivered in vivo by synthetic microspheres. Gene Therapy. 6: 1406-1414.
  8. Kazakov V. I., Mikhailov V.M. 2001. DNA fragmentation in cardiomyocytes of mdx and C57BL mice after dynamic stress. Tsitologiya. 43 (1) : 72-75.
  9. Mikhailov V.M., Komarov S.A., Nilova V. K. et al., 2001. Ultrastructural and morphometrical analysis of apoptosis of mouse mdx cardiomyocytes. Tsitologiya. 43 (8) : 729-734.
  10. Ilyina E. K., Vakhtin Yu.B. 2002. Molecular model for limb development. J. theor. Biol. 216: 159-177.
  11. Mironova A.P. 2002. The response of acclimated isolated gill epithelium of mollusk Anodonta Anatina L. to a short-term superoptimal heating. Tsitologiya. 44 (1) : 61-68.
  12. Mikhailov V.M., Kropotov A.V., Tomilin N.V. et al. 2002. Gene hACR-1 suppress a apoptosis of striated muscles M. quadriceps femoris of mdx mice after ballistic transfection. Tsitologiya. 44 (4).
  13. Mikhailov V.M. Kropotov A.V., Zelenin A.V. et al. 2002. The Bcl-xL and ACR-1 genes promote differentiation and reduce apoptosis in muscle fibers of mdx mice. Russ. J. Genetics. 38 (11) : 1221-1225.
  14. Mikhailov V.M. 2003. Life cycle of decidual cells. Inter. Rev. Cytol. 227: 1-63.
  15. Mikhailov V. M., Zhestyanikov V.D., Savelieva G.E. 2003. Myocard of mdx mice contains factor(s) that damage DNA structure and seem to retard DNA reparation after gamma-irradiation (experiences in modeling system). Tsitologiya. 45 (4): 418-421.
  16. Mikhailov V.M., Evtifeeva E.V., Serikov V.B., Perverzev A.E., Karmanova A.V., Zenin V.V. 2006. Participation of bone marrow stem cells in differentiation of mdx mice striated muscle. Tsitologiya. 48 (5): 410-417.
  17. Gavrilov B., Vezhenkova I, Mikhailov V. et al., 2006. Slow elimination of phosphorylated histon gamma-H2Ax from DNA of terminally differentiated mouse heart cells in situ. Biochem. Biophys. Research. Com. 347 : 1048-1052.
  18. Mikhailov V. M., Vezhenkova I.V., Gavrilov B.A. 2006. DNA reparation of mdx and C57BL/6 mice cardiac myocytes after dynamic stress and X-ray radiation. Acute cardiac care. 8 (Supp. 2) : 40-41.
  19. Mikhailov V.M., Vezhenkova I.V. 2007. Double-strand breaks of DNA of C57BL and mdx mouse cardiomyocytes after dynamic stress. Cell and Tissue Biology. 1 (4) : 328-333.
  20. Serikov B., Popov B., Mikhailov V. et al., 2007. Evidence of temporary airway epithelial repopulation and rare clonal formation by BM-derived cells following naphthalene injury in mice. Anat. Rec. 290 : 1033-1045.
  21. Alexeyeva N.P., Alexeyev A.O., Vakhtin Yu.B., et al., 2008. The reentrant binomial model of nuclear anomalies growth in rabdomyosarcoma RA-23 populations under increasing doze of rare ionizing radiation. Tsitologia. 50 (6): 528-534.
  22. Serikov V.B., Mikhaylov V.M., Krasnodembkay A.D., Matthay M.A. 2008. Bone marrow-derived cells participate in stromal remodeling of the lung following acute bacterial neumonia in mice. Lung. 186 : 179-190.
  23. Grinchuk T. M., Popov B.V.,Mikhailov V. M. et al.. 2008. Characterization of cultured murine mesenchymal stem cell line expressing GFP. Tsitologiya. 50 (12) : 1030-1035.
  24. Popov B.V., Petrov N.S., Mikhailov V.M. et al., 2009. Spontaneous transformation and immortalization of mesenchymal stem cells in vitro. Tsitologiya. 51 (2) : 91-102.
  25. Mikhailov V.M., Kaminskaja E.V., Popov V.B. et al., 2010. Characteristic of tumors developed after transplantation of transgenic GFP-positive C57BL/6 mice bone marrow mesenchymal stem cells to mdx mice muscle. Tsitologiya. 52 (10) : 853-857.
  26. Sokolova . A.V., Zenin V.V., Mikhailov V.M. 2010. Structure of neuromuscular junctions and differentiation of striated muscle fibers of mdx mice after bone marrow stem cells therapy. Tsitologiya. 52 (5) : 399-406.
  27. Arsen'ev A.V., Dudin M.G., Mikhailov V.M. 2010. Effect of the impulse magnetic field on the growth bone plates in experimental animals. Biofizika. 55 (4) : 699-703.
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