|Laboratory of Ion Channels of Cell Membranes|
Elena V. KAZNACHEYEVAD.Sci., Ph.D.
Phone: +7 (812) 297 14 97
Laboratory was established in 1983. From 1983 to 2005 its head was Corresponding Member of the Russian Academy of Sciences Professor G. N. Mozhayeva - one of the world's leading experts in the research of cell membrane ion channels. She is also one of the founders of this area of study in Russia.
The Laboratory Staff:
G. N. Mozhayeva - councilor of the Russian Academy of Sciences, Corresponding Member of the Russian Academy of Sciences
Research focuses on the molecular mechanisms of calcium signaling, with a particular interest in calcium signal transduction after activation of membrane receptors and issue
of calcium signal generation, which is the earliest cell response to receptor stimulus triggering many processes in cell. Laboratory has obtained new priority data proving
presence of the whole group of special-purpose calcium channels in the membrane of unexcitable cells. These channels perceive signals from receptors via GTF-binding
proteins as well as via water-soluble secondary messengers. The laboratory has discovered direct coupling and feedback taking place in the regulation of these channels' activity.
The receptors localized in endoplasmic membrane were shown to play a major role in activation of calcium channels. The hypothesis of direct protein-protein interactions between
calcium channels in the cytoplasmic membrane and channels in reticulum membrane (conformational coupling model) was proposed and confirmed experimentally.
Laboratory was first to reveal that products of a phosphoinositides exchange participate in regulation of calcium channels.
Various experimental approaches are used in studies: electrophysiology, molecular biology, calcium imaging using ion-sensitive fluorescent probes.
Research interests of the laboratory are currently directed toward studying mechanisms of calcium signaling abnormalities occurring in neuronal cells during the development of neurodegenerative diseases.
Work of the laboratory was supported by numerous grants from INTAS, CRDF, (Howard Hughes), Russian Foundation for Basic Research, Program of the Presidium of the Russian Academy of Sciences "Molecular and cell biology", Fund of assistance to a domestic science, grants of President of the Russian Federation and St. Petersburg Administration and Grants of the Federal target program "Scientific and scientific and pedagogical personnel of innovative Russia".
Naumov, A.P., Kaznacheyeva, E.V., Kiselyov, K.I., Kuryshev, Yu.A., Mamin, A.G., Mozhayeva, G.N. (1995) ATP-activated inward current and calcium-permeable channels in rat macrophages plasma membrane. Journal of Physiology (London) 486, 323-337.
Naumov, A.P, Kiselyov, K.I, Mamin, A.G, Kaznacheyeva E.V., Kuryshev Y.A, Mozhayeva G.N. (1995) ATP-operated calcium-permeable channels activated via a guanine nucleotide-dependent mechanism in rat macrophages. Journal of Physiology (London) 486, 339-347.
Naumov, A.P., Kaznacheyeva, E.V., Kuryshev, Yu.A., Mozhayeva, G.N. (1995). Selectivity of ATP-activated GTP-dependent Ca2+ -permeable channels in rat macrophage plasma membrane. J. Membr. Biol. 148, 91-95.
Kaznacheyeva, E.V., Mamin, A.G., Kiselyov, K.I., Mozhayeva, G.N., Naumov A.P. (1996) A New type of ATP-activated calcium channels in rat macrophages plasma membranes. Membr. and Cell Biol. 10 (2), 173-189.
Mamin A.G., Kiselyov K.I., Mozhayeva G.N. (1996). Effect of intracellular calcium on ATP-activated GTP-dependent calcium channels in rat macrophages. Journal of Physiology, (London). 491, 697-705.
Kiselyov K.I., Mamin A.G., Semyonova S.B., Mozhayeva G.N. (1997). Low conductance high selective inositol (1,4,5)-trisphosphate-activated Ca2+ channels in plasma membrane of A431 carcinoma cells. FEBS Letters. 407, 309-312.
Kaznacheyeva E.V., Lupu, V.D., Bezprozvanny I. (1998) Single-channel Properties of Inositol (1,4,5)-trisphsphate Receptor Heterologously expressed in HEK-293 Cells. J. Gen. Physiol. 111, 847-856.
Kiselyov, K., Xu, X., Mozhayeva, G., Kuo, T., Pessah, I., Mignery, G., Zhu, X., Birnbaumer, L., Muallem, S. (1998). Functional interaction between InsP3-receptor and store-operated Htrp3 channel. Nature. 396, 478-482.
Kiselyov, K.I., Mamin, A.G., Semyonova, S.B., Mozhayeva, G.N. (1999). Minitature Ca2+ chanels in excised plasma-membrane patches: activation by IP3. Pflugers Arch. 437, 305-314.
Zubov, A., Kaznacheyeva, E., Nikolaev, A., Alexeenko, V., Kiselyov, K., Muallem, S., Mozhayeva G. (1999) Regulation of the miniature plasma membrane Ca2+channel Imin by inositol 1,4,5-trisphosphate receptors. J. Biol. Chem. 274, 25983-25985.
Kaznacheyeva, E., Zubov, A., Nikolaev, A., Alexeenko, V., Bezprozvanny, I., Mozhayeva G. (2000) Plasma membrane calcium channels in human carcinoma A431 cells are functionally coupled to InsP3R-PIP2 complexes. J. Biol. Chem. 275, 4561-4564.
Kaznacheyeva, E., Zubov, A., Gusev, K., Bezprozvanny, I., Mozhayeva G. (2001) Activation of Ca2+ entry in human carcinoma A431 cells by Ca2+ store depletion and phospholipase C - dependent mechanisms converges on ICRAC-like channels. PNAS. 98 148-153.
Alexeenko V.A., Gusev K.O., Kaznacheeva E.V. (2003). Effect of readjustments of actin cytoskeleton on activation of inflow calcium channels in A431 cells. Tsitologiya. 45 (2): 143-148.
Gusev K., Glouchankova L., Zubov A., Kaznacheyeva E., Wang Z., Bezprozvanny I., Mozhayeva G. N. (2003) Store-operated calcium entry pathways in human carcinoma A431 cells: functional properties and activation mechanisms J. Gen. Physiol. 122 (1), 81-94.
Nikolaev A.V., Skopin A.Yu., Kaznacheeva E.V. (2004). Proteins of the Homer family regulate activity of Imin-channels in НЕК293 cells. Biol. Membrany, 21 (6) : 451-457..
Bugaj V., Alexeenko V., Zubov A., Glushankova L., Nikolaev A., Wang Z., Kaznacheyeva E., Bezprozvanny I., Mozhayeva G.N. (2005) Functional properties of endogenous receptor- and store-operated calcium influx channels in HEK293 cells. J. Biol. Chem. 280, 16790-16797.
E. N. Kaznacheeva, L. N. Glushankova, V. V. Bugaj, O. A. Zimina, A. Yu. Skopin, V. A. Alexeenko, I. B. Bezprozvanny, G. N. Mozhayeva (2007) Role of TRPC3 in the Formation of Receptor- and Store-operated Calcium Channels in A431 Carcinoma Cells. Biol. Membrany. 24(1), 90-99.
Kaznacheyeva E., Glushankova L., Bugaj V., Zimina O., Skopin A., Alexeenko V., Tsiokas L., Bezprozvanny I., Mozhayeva G.N. (2007) Suppression of TRPC3 Leads to Disappearance of Store- operated Channels and Formation of a New Type of Store-independent Channels in A431 Cells. J. Biol. Chem. 282(32), 23655-23662.
O. A. Zimina, L. N. Glushankova, A. Yu. Skopin, V. A. Alexeenko, V. A. Vigont, G. N. Mozhayeva, E. V. Kaznacheyeva (2008) The Role of STIM1 Protein in the Regulation of Calcium Entry into A431 Human Epidermoid Carcinoma Cells. Doklady Biological Sciences, 420: 214-217.
O. A. Zimina, V. A. Vigont, I. A. Pozdnjakov, L. N. Glushankova, S. V. L'vovskaja, A. Yu. Skopin, G. N. Mozhayeva, E. V. Kaznacheeva (2010) Role of STIM1 in the Receptor and Store Operated Calcium Influx in HEK293 Cells. Biol. Membrany. 27(3), 237-243.
Shalygin A., Ryazantseva M., Glushankova L., Mozhayeva G., Bezprozvanny I., Kaznacheyeva E. (2010) Homer regulation of native plasma membrane calcium channels in A431 cells. Cell Calcium. 48, 209-214/
Glushankova LN, Zimina OA, Vigont VA, Mozhaeva GN, Bezprozvanny IB, Kaznacheeva EV. (2010) Changes in the store-dependent calcium influx in a cellular model of Huntington's disease . Dokl Biol Sci. 433:293-5.